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How are structures formed in a LAMP assay?
The structures are formed by annealing between alternatively inverted repeats of the target sequence in the same strand. In a LAMP assay, the reaction takes place in a single tube containing buffer, target DNA, DNA polymerase and primers.
How are target sequences amplified in lamp amplification?
Thus, a LAMP target sequence is amplified 13 fold every half cycle. The final products obtained are a mixture of stem loop DNA with various stem lengths and various cauliflower like structures with multiple loops. The structures are formed by annealing between alternatively inverted repeats of the target sequence in the same strand.
What does lamp stand for in amplification technology?
"LAMP" stands for Loop-mediated Isothermal Amplification. This technology was developed by Notomi et al. It is a very sensitive, easy and time efficient method. The LAMP reaction proceeds at a constant temperature using a strand displacement reaction.
Last Update: Oct 2021
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Keyerra
25.10.2021 06:23
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